![]() Now you create a statistic that tells you the median forward scatter signal for "Live cells." Thus, you create a new node (analysis) attached to the "Live cells" gate of the first sample. After looking at the 3 samples, youįind that you have to alter the position of the gate slightly for each one-therefore, the gates are no longer identical. You first decide on a gate to exclude dead cells and clumps of cells you apply this gate to a group containing all three samples (and call it "Live cells"). ![]() During batch operations, you can specify that this analysis be applied to all samples within a group.įlowJo decides how to do this by looking at the names of the gates.įor instance, you may have 3 samples in a workspace. When you specify a kind of analysis (for instance, a statistical measurement, or a gate selecting a subset of cells), it is applied to a population. Gates are similar to what we expect for rules for naming people in families: siblings cannot have the same name however, different generations can have gates with the same name. Naming populations is very important for the way in which FlowJo works. FlowJo identifies populations (samples, or gated subsets of samples) by a name that you assign.
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